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Scientist - III (Senior)
Ref No.: 18-12413
Location: West Point, Pennsylvania
Job description • Conduct hands-on purification research in the Department of Infectious Diseases and Vaccines Vaccine according to project timelines and priorities. • Design and run experiments to develop purification processes and produce purified biomolecules suitable for vaccine research. • Develop scalable purification processes for recombinant proteins by affinity, ion-exchange, and size-exclusion chromatography. • Collaborate with various groups by providing purification, characterization, and expression support. • Characterize purified products by electrophoresis, densitometry, Western blotting, protein concentration assays, endotoxin assays, absorbance spectroscopy, and HPLC. • Perform protein expression in various expression systems (mammalian, insect cell, bacterial). • Document experimental results in electronic notebook format. • Prepare and present regular progress reports to managers. • Maintain a current understanding of protein chemistry literature and methodology, as well as other scientific literature related to the specific vaccine discovery projects.

Quals--
Required • A degree in biochemistry or similar discipline. • Experience with various separation technologies including chromatography, centrifugation and filtration. • Research experience with protein purification, purification process development, and process optimization. • Experience with electrophoresis, densitometry, Western blotting, protein concentration assays, endotoxin assays, absorbance spectroscopy, and HPLC. // Preferred • Experience in discovery research in an industrial setting. • Experience with cell culture techniques, mammalian expression, and/or other expression systems. • Experience in sterile technique and safe handling of infectious BSL2 organisms. • Experience with AKTA Purifier or Bio-Rad LC systems. • Experience with Waters EMPOWER chromatography systems.