Ref No.: 19-16251
Location: Waltham, Massachusetts
Sunrise System Inc. is currently looking for Scientist in Waltham, MA with one of our top client.
Job Title: Scientist
Job Id: 19-16251
Location: Waltham, MA 02451
Duration: 12 months
Position Type: Hourly contract Position (W2 only)
 
Duties
  • We are seeking a flow cytometry core technologist to join our hematology research team developing therapies for haemophilia, sickle cell disease, and other non-malignant haematological disorders.
  • The successful candidate will be responsible for independently setting up a range of flow-based experiments that include routine flow cytometry, complex highly multi-parametric analyses, hematopoietic single cell sorting, and other specialized cell characterization methods.
  • The applicant will be responsible for maintaining flow cytometry equipment and training users on the proper design of experiments, use of equipment and correct data analysis.
  • The qualified candidate should have a desire to build on their existing knowledge of cell phenotyping, and a willingness to take on new challenges toward the goal of building a state-of-the-art flow cytometry lab. 
Responsibilities
  • Perform cell sorting and manage sort requests.
  • QC instruments on a daily basis and troubleshoot problems; build and change cytometer configurations as needed.
  • Develop and perform multi-color staining assays for primary human and mouse phenotyping experiments.
  • Characterize and qualify reagents to be used in analysis and sorting assays, including FACS antibody panels and titers.
  • Perform data analysis using FlowJo and FACSDiva applications when needed.
  • Help train other users on all aspects of flow cytometry from planning experiments, acquisition and analysis.
  • Interpret and summarize experimental data and report summaries in team meetings, as needed.
  • Experience working in or running a core facility is a plus.
  • Experience running high content microscopy platforms, including Perkin Elmer Operetta and EMD
  • Millipore Image Stream, and associated data analysis is a plus.
  • Experience with or a willingness to be trained on the operation of hematology analyzers (Advia, Beckman Coulter) is a plus.
  • Well versed in a variety of cell based methods with a strong understanding of tissue culture, cell biology, immunoassays, and general laboratory techniques.
Qualifications
  • PhD degree with 5+ years flow cytometry experience in appropriate scientific area related to position.
  • A degree in cell biology, immunology, molecular biology, microbiology, or biochemistry is preferred.
  • Have an understanding of flow cytometry fundamentals and experience with analyzers including: Client LSR II, Canto II and Guava EasyCyte HT.
  • Experience cell sorting on a Client FACS Aria Fusion
  • Comfortable developing and running 6+ color FACS panels.
  • General ability working with Windows and MAC operating systems and computer hardware/devices.
  • Proficiency with the following software: Microsoft Office, FACS Diva, FlowJo, MACS Quantify, Forecyte and Graph pad Prism.
Skills and Experience
  • Flow cytometry
  • Hematology 
Additional Skills
  • Sorting 
Contact
Chenchukala Alwar
Direct: 732-313-0243| Email: Chenchukala.A@sunrisesys.com
Or
Satya S
Direct: (732) 272.0262 | Email: Satya@sunrisesys.com